rat col i elisa kits  (Cusabio)

Journal: Molecular Biology Reports

Article Title: Silymarin and MSC-exosomes ameliorate thioacetamide-evoked renal fibrosis by inhibiting TGF-β/SMAD pathway in rats

doi: 10.1007/s11033-024-09343-6

Figure Lengend Snippet: RT-PCR expression levels of TGF-β receptors and ELISA expression levels of the studied proteins. ( a ) RT-PCR expression levels of TβRI; ( b ) RT-PCR expression levels of TβRII. ( c ) ELISA expression levels of collagen type I protein; ( d ) ELISA expression levels of CTGF protein. Data are expressed as mean ± SD; a p-value < 0.05 is significant; (*) vs. the control group, (#) vs. the TAA group, and ($) vs. the TAA + SM group all denote a significant difference

Article Snippet: The kidney homogentisate was used to measure the expression levels of collagen type I using the CUSABIO Col I ELISA Kit (#Cat No. CSB-E08084r), and CTGF proteins using the MyBioSource ELISA Kit (#Cat No. MBS261004), according to the manufacturer’s protocols.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production

doi: 10.3390/ijms222313089

Figure Lengend Snippet: Col I/III production from co-cultures with and without mechanically loaded myoblasts. ( A , D ) Relative mRNA expression of Col 1a1 and Col 3a1 in tenocytes. ( B , E ) Quantification of Col 1 content in tenocyte lysate by ELISA. ( C , F ) Quantification of Col I/III content and the ratio in the cell culture medium. ( G ) Immunofluorescence staining of Col I on tenocytes. Teno ctrl: tenocytes alone, Co ctrl: tenocytes co-cultured with myoblasts, Dyn: tenocyte co-cultured with dynamically loaded myoblasts, Stat: tenocyte co-cultured with statically loaded myoblasts. Data are represented as mean ± standard deviation. n = 3. Student’s t -test was performed when comparing Teno ctrl and co ctrl. One-way ANOVA with Tukey’s multiple comparisons was performed when comparing between Co ctrl, Dyn and Stat groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Collagen I and collagen III were assessed using Rat Collagen Type I ELISA kit (Cusabio, Wuhan, China, #CSB-E08084r) and Rat Collagen Type III ELISA kit (Cusabio, Wuhan, China, #CSB-E07924r) according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Standard Deviation